Modifying Enzymes
Worthington Biochemical Corporation COLLAGENASE TYPE 4 50 MG
Collagenase, Type 4 (Code: CLS-4), which is filtered through a 0.22 micron membrane and lyophilized in vials.
Bioworld Lysozyme, 5 g
Lysozyme Enzyme used in purification protocols of genomic and plasmid DNA to lyse bacterial cells.Ultra Pure GradeSource: Hen Egg White (also called Hen Egg Lysozyme or HEL.)Activity(25 C): >20,000U/mgAlbumin: None detectedSalmonella: None detected
Bioworld Lysozyme, 10 g
Lysozyme Enzyme used in purification protocols of genomic and plasmid DNA to lyse bacterial cells.Ultra Pure GradeSource: Hen Egg White (also called Hen Egg Lysozyme or HEL.)Activity(25 C): >20,000U/mgAlbumin: None detectedSalmonella: None detected
New England Biolabs, Inc. Thermostable OGG - 500 units
Thermostable OGG is an archaeal 8-oxoguanine (8oxoG) DNA glycosylase which acts both as aN-glycosylase and an AP-lyase. TheN-glycosylase activity releases the damaged purine (8-oxoguanine) from double stranded DNA, generating an apurinic (AP) site. The AP-lyase activity cleaves 3' to the AP site leaving a 5' phosphate and a 3'-phospho-alpha, beta-unsaturated aldehyde. Unlike some other DNA glycosylases, Thermostable OGG specifically recognizes and cleaves only 8oxoG and no other modified bases.
Rockland Immunochemicals Protease S. aureus 1 mg
Protease S. aureus V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27 000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease S. aureus V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F- Cl- CH3COO- and NO3. Enzyme activity is determined by the casein digestion assay. Protease S. aureus is ideal for investigators involved in enzyme and infectious diease research.
New England Biolabs, Inc. Thermostable Inorganic Pyrophosphatase – 250 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 + H2O -> 2HPO4-2.
INTACT GENOMICS INC Endonuclease IV (Nfo) 5000 Units (10 units/µl)
Endonuclease IV (Nfo) from Escherichia coli is a 32-kD metalloprotein that aids in the repair of damaged DNA. The enzyme functions both as an apurinic/apyrimidinic nuclease (1) and as a 3′-terminal di-esterase (1-4). Its 3′-terminal di-esterase activity is important in the repair of DNA strand breaks generated by oxidation and ionic radiation (2, 3). In such events, the strand breaks terminate with either a 3′ phosphate or a deoxyribose fragment, preventing repair by DNA polymerase I or DNA ligase. Endonuclease IV removes the blocking groups, leaving a free 3′-hydroxyl terminus. This enzyme does not have detectable associated exonuclease or DNA N-glycosylase activity (1).Applications• Single cell gel electrophoresis (Comet assay) (5, 6)• Alkaline elution (7)• Alkaline unwinding (8)Product SourceE. coli BL21 (DE3) strain expressing E. coli Endonuclease IV gene.Product Includes• Endonuclease IV (Nfo)• 10x Endonuclease IV reaction buffer
Enzyme Research Laboratories Inc BOVINE FACTOR XA 1MG BOVINE FA
Bovine Factor Xa is prepared from BFX by activation with Russell's Viper Venom. Complete activation is observed by SDS-PAGE.
New England Biolabs, Inc. T4 Polynucleotide Kinase (3' phosphatase minus) – 200 units
T4 Polynucleotide Kinase catalyzes the transfer and exchange of P from the gamma position of ATP to the 5 -hydroxyl terminus of double- and single-stranded DNA and RNA, as well as nucleoside 3'-monophosphates. This modified version exhibits full kinase activity with no 3' phosphatase activity.
INTACT GENOMICS INC T4 DNA Ligase 100,000 Units (2000 units/µl)
Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzyme joins DNA fragments with either cohesive or blunt termini and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).Applications• Cloning of restriction enzyme generated DNA fragments• Cloning of PCR products• Next-gen library preparation• Joining linkers and adapters to cohesive or blunt-ended DNA• Nick repair in duplex DNA, RNA or DNA/RNA hybrids• Self-circularization of linear DNA
Carnabio Usa Inc BTN - EGFR[T790M/L858RÙ/ 10UG
BTN-EGFR[T790M/L858R], Cytoplasmic domain, Wild type, Amino Acid 669-1210(end), NP_005219.2, 66 kDa, Insect (sf21); 10microg
GeneCopoeia BIOTIN PROTEIN LIGASE
Biotin Protein Ligase - 40 ug: 2 vials of 20 ug each (200,000 Units)
New England Biolabs, Inc. Thymine-DNA Glycosylase (TDG)-500 units
Thymine-DNA glycosylase (TDG) catalyzes the hydrolysis of the N-glycosidic bond of thymine or uracil mispairs opposite guanine that arise from spontaneous deamination events of 5-methylcytosine (5mC) and cytosine, respectively.The excision of the mispaired thymine or uracil base leaves an abasic (AP) site that can then be chemically or enzymatically cleaved.
- Active on dsDNA
- Monofunctional glycosylase that removes dT or dU nucleobases mispaired with dG to create an AP site
- Removes 5-formylcytosine, 5-carboxylcytosine, 5-hydroxymethyluracil, ethenocytosine, 5-fluorouracilIsolated from a recombinant source
- Supplied with 10X TDG Reaction Buffer